The MIT Huang-Hobbs BioMakerSpace

About the space

The MIT Huang-Hobbs BioMaker Space is a BL2 biological lab located in the basement of Building 26, Room 26-035. It includes the essential equipment for most biological and chemical engineering projects. Adjacent to the lab is a lounge area in 26-033 where students can work and hang out.

General

  • Vortexers, shakers, stirrers, nutators
  • Minifuges, microfuges, table-top centrifuges
  • BioRad T100 thermocycler
  • qPCR machine
  • DNA agarose gel electrophoresis
  • Spectrophotometer (UV/Vis)
  • Gel transilluminators (blue light, UV)
  • Electroporator
  • Balance/scales
  • Vacuum oven

Cell culture

  • Anaerobic glove box
  • 37ºC incubator
  • 30ºC incubator
  • Biosafety cabinets
  • Fermenter/bioreactor
  • 37ºC incubator with 5% CO2
  • Neubauer-improved hemocytometers

Chemical synthesis

  • Chemical fume hoods
  • Rotovap

Protein analysis

  • Ultrasonic sonicator
  • Protein purification (affinity column, FPLC)
  • BioRad SDS-PAGE protein gel electrophoresis

Fabrication

  • Power supply, signal generator, oscilloscope
  • Circuit board mini desktop CNC mill
  • Soldering station
  • Arduinos
  • SLA Resin 3D printer
  • FDM Filament 3D printer
  • Microfluidics/PDMS equipment
  • Spincoater

Measurement

  • Keyence BZ-X810 microscope (brightfield, phase, fluorescence)
  • Sony SH800 Fluorescence-activated cell sorter
  • Agilent BioTek Synergy H1 plate reader
  • Atomic-force microscope (AFM)

Utility

  • Autoclave
  • Milli-Q water
  • Ice machine
  • Dishwasher

Storage

  • 4ºC fridge
  • -20ºC freezer
  • Ultra low-temperature (-80ºC) freezer
  • Vapor-phase liquid nitrogen storage
  • Room-temperature shelves

Independent projects

Have an independent project (i.e. unrelated to UROP, PI research, or sponsored research) that you'd like to perform in the space? Fill out our two-stage application to get approved to use the space!

Note that EHS approval takes time (at least one month), so submit your application as soon as possible to get the process started.